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KMID : 1037020140030010027
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Medical Lasers; Engineering, Basic Research, and Clinical Application
2014 Volume.3 No. 1 p.27 ~ p.30
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Effect of Photodynamic Therapy in Melanoma Skin Cancer Cell Line A375: in vivo Study
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Chung Phil-Sang
Ahn Jin-Chul
Lee Sang-Joon
HE Peijie
Moon Jeong-Hwan
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Abstract
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Background and Objectives: Aminolevulinic acid (ALA), a precursor of heme, which is converted to protoporphyrin IX, is used as an effective photosensitizer. The aim of this study is to evaluate the effect of photodynamic therapy (PDT) using 5-ALA in an in vivo experiment using melanoma skin cancer cell line A375.
Materials and Methods: Female nude mice (BALB/C/nu/nu), four weeks of age and 25-30 g (Narabio, Korea) in weight, were used in the study. When the actual tumor size reached more than 100-200 mm3, the mice were divided randomly into the PDT group (n = 7), LED (light emitting diode) group (n = 3), ALA group (n = 3), and control group (n = 7). ALA 375 mg/kg was used in the study as an intraperitoneally injected photosensitizer. The tumors were then irradiated using a 630 nm LED for 30 min after 3 hours in a dark room. Tumor dimensions were measured using a caliper at 0, 7, 14, 21, and 28 days after treatment in all groups and tumor volume was calculated.
Results: There was no significant difference in the mean tumor volumes on 0 day before treatment in each group (p>0.05). However, significant differences in the mean tumor volumes were observed between the PDT and control groups at 7, 14, 21, and 28 days after treatment (p<0.05).
Conclusion: ALA mediated PDT was found to be effective in melanoma skin cancer cell A375 induced tumor xenograft in nude mice.
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KEYWORD
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Skin cancer, Melanoma, Photodynamic therapy, Aminolevulinic acid
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